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primary antibody incubation  (Biorbyt)


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    Biorbyt primary antibody incubation
    Primary Antibody Incubation, supplied by Biorbyt, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/antibody/pm41826998-136-0-10
    Average 96 stars, based on 643 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Interpretation of Cellular Imaging and AQP4 Quantification Data in a Single Cell Simulator
    Article Snippet: .. Primary antibody specific for Nrf2 phosphorylated at serine 40 site was purchased from Biorbyt and primary antibody incubation was carried out overnight. .. On the next day, samples were incubated with secondary anti-rabbit antibody conjugated to Alexa594 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and subsequently stained with DAPI.

    Article Title: Response gene to complement 32 promotes tumorigenesis by mediating DNA damage repair and inhibits CD8+ T cells infiltration in diffuse large B-cell lymphoma
    Article Snippet: .. Primary antibody incubation included anti-RGC32 (orb2372, Biorbyt, 1:200) and species-specific CD8a antibodies (human: A0663, ABclonal 1:200; murine: GB114196, Servicebio 1:500) at 4°C overnight, supplemented with anti-Ki67 (GB121141, Servicebio 1:300) for murine specimens. ..

    Article Title: Telomere damage-mediated senescence in alveolar epithelial type II cells but not in macrophages aggravates inflammation in acute lung injury.
    Article Snippet: .. Primary antibody incubation was performed with goat anti-tdTomato antibody (orb182397, Biorbyt) 1:250 diluted in the blocking solution for 60 minutes. .. After washing, slides were incubated with the secondary antibody Alexa Fluor 594 donkey anti-goat (A11058, Invitrogen) 1:500 diluted in blocking solution for 60 minutes.

    Blocking Assay:

    Article Title: Telomere damage-mediated senescence in alveolar epithelial type II cells but not in macrophages aggravates inflammation in acute lung injury.
    Article Snippet: .. Primary antibody incubation was performed with goat anti-tdTomato antibody (orb182397, Biorbyt) 1:250 diluted in the blocking solution for 60 minutes. .. After washing, slides were incubated with the secondary antibody Alexa Fluor 594 donkey anti-goat (A11058, Invitrogen) 1:500 diluted in blocking solution for 60 minutes.



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    Image Search Results


    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Journal: bioRxiv

    Article Title: Neural cell state modulation by PARK2 and dopaminergic neuroprotection by small molecule Parkin agonism

    doi: 10.64898/2026.04.01.715918

    Figure Lengend Snippet: ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Article Snippet: Membranes were incubated with primary antibodies overnight at 4°C following blocking with 5% Bovine Serum Albumin (Millipore Sigma, A9647-100G) in TBS with Tween (Thermo Scientific Chemicals, J77500.K2) for 1 h. Primary antibody incubation was done overnight, while secondary antibody incubation was done at room temperature for 1 h. Membranes were imaged using ChemiDoc (Bio-Rad) and analyzed using Image Lab software (Bio-Rad).

    Techniques: Staining, Derivative Assay, Imaging, Incubation, Control, Western Blot, Transmission Assay, Electron Microscopy, Recombinant, Sonication

    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Nutrition

    Article Title: Lactobacillus acidophilus alleviates slow transit constipation by modulating 5-HT pathway and gut microbial composition

    doi: 10.3389/fnut.2026.1775405

    Figure Lengend Snippet: Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Primary antibody incubation against TNF-α (Proteintech, Wuhan, China) (60291-1-IG) (dilution 1:1000) was performed overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Quantitative RT-PCR